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Key Performances of immunoassay using the described antibodies and conjugates

• LOD <50pg/mL by P3dG-DELFIA

• The McAb shows 2.9% cross-reactivity with estrone. The cross-reactivities were less than 0.1% for estrone-3-sulfate, estradiol and estriol

Typical calibration curves for P3dG-DELFIA

Certificate of analysis

Pregnanediol-3-glucuronide (P3dG) Mouse Monoclonal Antibody

• Catalog Number: MA-P3dG-10#, MA-P3dG-51#  

• Clonality: Monoclonal

• Clones: 10#, 51#

• Immunogen: Pregnanediol-3-glucuronide-BSA

• Source: Hybridoma cell

• Intended use: Immunoassays

 Supplied as: µg or mg of Antibody (purified)

• Buffer: PBS

• Storage: 2-8°C

• Concentration: As specified on label

• Expiration Date: As specified on label

Pregnanediol-3-glucuronide (P3dG) -PEG-Protein conjugate

• Catalog Number: P3dG-PEG-Protein 0319#

• Source: Chemical synthesis

• Intended use: Paired with MA-P3dG McAb for P3dG immunoassay

• Supplied as: µg or mg of conjugates

• Buffer: PBS

• Storage: 2-8°C

• Concentration: As specified on label

• Expiration Date: As specified on label

 

 

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Product information

Antibodies and conjugates for immunoassay of Pregnanediol-3-glucuronide 


Pregnanediol-3-glucuronide

CAS registry No.: 1179-77-9

Formal Name: (5β)-Pregnan-3α,20α-diol 3-glucuronide

FW: 494.62  

MF: C27H42O8



About the immunoassay of Pregnanediol-3-glucuronide

The determination of serum level of 17β-estradiol (E2) and progesterone (P) by immunoassay has accepted as a reliable method for monitoring ovulation induction and in vitro fertilization. However, this method is poorly accepted by patients because of need of frequent clinic visits for repetitive and painful blood sampling.

Compared with the blood sampling, immunoassay of urine E3G (Estrone-3-glucuronide) and P3dG (Pregnanediol-3-glucuronide) provides a practical alternative for rapid, accurate and simple fertility monitoring due to the non-invasive and more convenient urine sampling. It makes the designing of POCT devices for ovulation monitoring more feasible, especially when  combined with creatinine correction of the E3G and P3dG concentration.

By Wu FB, PhD, Mb-15221067868

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